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Luciferase Reporter Gene Detection Kit

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NACRES:

NA.84

UNSPSC Code:

12352207

Usage:

kit sufficient for 100 assays

Storage temp.:

−70°C

Pricing and availability is not currently available.

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Properties

usage

kit sufficient for 100 assays

Quality Segment

200

storage temp.

−70°C

Description

General description

Firefly luciferase is one of the most utilized reporter genes for the study of gene expression. It is an extremely sensitive, rapid, and easy-to-use reporter gene. The chemiluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. The luciferase substrate contains coenzyme A for increased and sustained luminescence compared to conventional methods. This eliminates the need for automated luminometer injection of the substrate and allows analysis by photographic film or scintillation counting. The lysis buffer contains polymyxin B which further enhances the signal and eliminates lysozyme treatment and freeze-thawing of cells. Cell lysis buffer is compatible with β-galactosidase assays. The enzyme encoded by the luciferase reporter gene catalyzes the oxidation of D-luciferin in the presence of ATP, oxygen, and Mg²⁺. The fluorescent product is quantified to measure its activity.

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This Item	SCT150	SCT151	L9420
Sigma-Aldrich LUC1 Luciferase Reporter Gene Detection Kit	Sigma-Aldrich SCT150 Firefly Luciferase HTS Assay	Sigma-Aldrich SCT151 Firefly Luciferase Assay (Lyophilized)	Sigma-Aldrich L9420 Luciferase from Photinus pyralis (firefly)
description -	description Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours	description Lyophilized flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.	description recombinant, expressed in E. coli, buffered aqueous solution, ≥10×10¹⁰ units/mg protein
usage kit sufficient for 100 assays	usage -	usage -	usage -
Quality Level 200	Quality Level -	Quality Level -	Quality Level 200
storage temp. −70°C	storage temp. -65 to -96°C	storage temp. -10 to -25°C	storage temp. −20°C