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Invertase from Candida utilis

Name: Invertase from <I>Candida utilis</I>
Brand: SIGMA

Grade X, ≥300 units/mg solid

Synonym(s):

β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase

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About This Item

CAS Number:

9001-57-4

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-615-7

MDL number:

MFCD00131410

EC Number:

3.2.1.26 (BRENDA, IUBMB)

Specific activity:

≥300 units/mg solid

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Properties

type

Grade X

Quality Segment

200

form

solid

specific activity

≥300 units/mg solid

mol wt

~260 kDa

foreign activity

α-galactosidase (melibiase), essentially free

storage temp.

−20°C

Description

General description

Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae.

Michaelis constant : 1.5x10 ^-2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70o^C
pH Stability : pH 4.0 - 6.0 (50o^C, 10min)
Thermal stability : below 60o^C (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6⁾

Application

Invertase from Candida utilis has been used:

as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp.
to determine the sucrose content in ethanolic extract of leaf tissues
to determine the total non-structural carbohydrates (TNC) in root tissues

This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.

Biochem/physiol Actions

Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries.

Preparation Note

Chromatographically purified

Other Notes

One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.

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This Item	I4504	I9274	I0408
Sigma-Aldrich I4753 Invertase from Candida utilis	Sigma-Aldrich I4504 Invertase from baker′s yeast (S. cerevisiae)	Supelco I9274 Invertase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich I0408 Invertase Glycoprotein Standard
specific activity ≥300 units/mg solid	specific activity ≥300 units/mg solid	specific activity 200-300 units/mg solid	specific activity -
form solid	form solid	form -	form lyophilized powder
mol wt ~260 kDa	mol wt -	mol wt -	mol wt 60 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
foreign activity α-galactosidase (melibiase), essentially free	foreign activity α-galactosidase (melibiase) ≤0.01%	foreign activity α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%, 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%	foreign activity -
Quality Level 200	Quality Level 300	Quality Level 100	Quality Level 200

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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