I3265

Interferon-γ human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), lyophilized powder, suitable for cell culture

• Synonym(s): IFN-γ
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00131391

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• mol wt: ~17 kDa
• packaging: pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg endotoxin
• color: white
• solubility: water: soluble 1.000 mL, clear, colorless
• UniProt accession no.: P01579
• storage temp.: −20°C
• Gene Information: human ... IFNG(3458)

Description

Application

Interferon-γ human has been used:

• to study the effect of interferon-γ on cultured trophoblasts in absence and presence of fibronectin
• to analyse factors which participate in the production of antibody microarrays on glass support
• for ELISA (enzyme-linked immunosorbent assay) method to understand the effect of interferon-γ in human gingival fibroblasts in presence of bacterial challenge

Biochem/physiol Actions

IFNG (interferon-γ) is a type-2 interferon which is produced by Th (T helper cells)-1 lymphocytes, CD8 cytotoxic lymphocytes, natural killer (NK) cells, and natural killer T (NKT) cells. It plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. High expression of IFNG is common in many autoimmune diseases, such as systemic lupus erythematosis, Sjögren syndrome, inflammatory bowel disease and multiple sclerosis.

Physical form

Lyophilized from phosphate buffered saline.

Analysis Note

The biological activity is measured by a viral resistance assay.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)