electron microscopy: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable indirect ELISA: suitable western blot: 1:400 using cultured human foreskin fibroblast extract
Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.
Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.
Specificity
The antibody recognizes an epitope located between amino acid residues 383-447 of the human HSP 60. It is not cross-reactive with the bacterial counterpart (E. coli), or with helminth and spinach HSP60. The antibody reacts with constitutive and inducible HSP60. It demonstrates a raised level of staining in immunohistochemistry of synovial membranes taken from patients with juvenile chronic arthritis.
Immunogen
recombinant human heat shock protein 60 (HSP60)
Application
Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:
Enzyme linked immunosorbent assay (ELISA)
Immunoblotting
Immunocytochemistry
Immunohistochemistry
Immunoelectron microscopy
Double immunofluorescence
Biochem/physiol Actions
A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.
Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.
Physical form
Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequency of adenocarcinoma has been reported in patients with COPD. Heat shock proteins (Hsps) are implicated in tumoral cell
Cell death and differentiation, 9(1), 82-88 (2002-01-23)
As shown here, mitochondria purified from different organs (liver, brain, kidney, spleen and heart) contain both pro-caspase-9 and the processed, mature form of caspase-9. Purified liver mitochondria release mature caspase-9 upon induction of permeability transition in vitro. This is accompanied
Hsp60 and Hsp10 increase in colon mucosa of Crohn?s disease and ulcerative colitis
Rodolico V, et al.
Cell Stress & Chaperones, 15(6), 877-884 (2010)
Studies characterizing 60 kda autoantibodies in subjects with schizophrenia
Wang X, et al.
Biological Psychiatry, 53(5), 361-375 (2003)
HSP60: issues and insights on its therapeutic use as an immunoregulatory agent
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