H0786

Heregulin-β, EGF Domain human

≥80% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• Synonym(s): HRG-β
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01867052

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: 185 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• UniProt accession no.: Q02297
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... NRG1(3084)

Description

General description

Truncated human sequence (amino acids 178-241) corresponding to the EGF domain and purified as a GST-fusion protein, cleaved with thrombin.

Biochem/physiol Actions

Growth factor ligand for ErbB3 and ErbB4 receptor tyrosine kinases.

Growth factor ligand for ErbB3 and ErbB4 receptor tyrosine kinases. The binding of HRG results in receptor dimerization and receptor trans-autophosphorylation. The phosphorylated receptors recruit cellular signaling proteins, initiating signaling pathways.

Physical form

Solution in phosphate buffered saline containing 30% glycerol.

Analysis Note

Stimulates tyrosine phosphorylation of the ErbB-3/HER-3 receptor.

The biological activity is measured by its ability to activate the c-ErbB3/HER-3 receptor in treated MCF-7 cells.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable