CRISPR18V
Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)
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About This Item
NACRES:
NA.51
UNSPSC Code:
41106609
Form:
liquid
Concentration:
(1x106 TU/ml (via p24 titering assay))
Pricing and availability is not currently available.
form
liquid
Quality Segment
packaging
vial of 200 μL
concentration
(1x106 TU/ml (via p24 titering assay))
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro#1 usesl a dual-component system consisting of U6-driven guide RNA (non-target#1) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Application
Functional Genomics/Screening/Target Validation
Other Notes
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.
Legal Information
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description - |
| form liquid | form liquid | form liquid | form liquid |
| concentration (1x106 TU/ml (via p24 titering assay)) | concentration (1x106 TU/mL (via p24 titering assay)) | concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA ) | concentration (1x106 TU/mL (via p24 titering assay)) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −70°C | storage temp. −70°C | storage temp. −20°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| application(s) CRISPR | application(s) CRISPR | application(s) CRISPR | application(s) CRISPR |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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