CRISPR11V
CRISPR-Lenti Human EMX1 Positive Control Transduction Particles
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About This Item
UNSPSC Code:
41106609
NACRES:
NA.51
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form
liquid
packaging
vial of 200 μL
concentration
1x106 VP/ml (via p24 assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The Sigma CRISPR-LENTI HUMAN EMX1 POSITIVE CONTROL TRANSDUCTION PARTICLES are a critical positive control to monitor transduction efficiency for lenti CRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human EMX1. EMX1 is one of the members of the EMX homeobox family, which are expressed in the developing cerebrum and involved in specification of positional identity and neural cell proliferation. This targets a validated EMX1 CRISPR site, which serves as an experimental control for Sigma lenti CRISPRs. The Sigma lenti CRISPR positive control is a one-component system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA plasmid targeting the human EMX1 gene, with both Puromycin and GFP co-expressed with Cas9.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
Application
Functional Genomics/Screening/Target Validation
To see more application data, protocols, vector maps visit sigma.com/crisprs.
- Positive control, CRISPR lentivirus, viral format, for creating gene knockouts/genetic modifications in cell lines.
To see more application data, protocols, vector maps visit sigma.com/crisprs.
Features and Benefits
Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPRs (virus format). Allows for validation of your system with the lenti CRISPR/Cas9 system. A positive result in a mis-match detection assay will indicate validation of your system.
Physical form
200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
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