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product name
F5-5.F1, 99072803
biological source
mouse spleen
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Lymphocyte, friend leukemia
Cell Line Description
Investigation of differentiation-inducible leukaemia cells is considered important in the understanding of leukaemic or neoplastic state. Several ascites lines derived from friend virus-induced erythoid leukemia show an erythroid morphology and also, unusually high haem synthesis. Most cultured lines of friend virus-induced leukemia in mice, are known to be inducible to differentiate towards erythocytes. F-5-5.F1 is susceptible to the induction by both dimethyl and butyric acid.
Culture Medium
RPMI 1640 + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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