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C8616

Sigma-Aldrich

Monoclonal Anti-phospho-β-Catenin (pThr41) antibody produced in mouse

clone BCT-41, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BCT-41, monoclonal

form

buffered aqueous solution

mol wt

antigen ~94 kDa

species reactivity

human

concentration

5-10 mg/mL (IgG fraction of antiserum)

technique(s)

immunocytochemistry: 1:50 using 293 T (human embryonic kidney cell)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 8-10 μg/mL using extract of cultured human 293T cells treated with Lici (20 mM) and calyculin A (50 nM)

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr41)

Gene Information

human ... CTNNB1(1499)

General description

Monoclonal Anti-phospho-β-Catenin (pThr41) (mouse IgG1 isotype) is derived from the BCT-41 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene.

Specificity

Monoclonal Anti-phospho-β-Catenin (pThr41) reacts specifically with human β-catenin phosphorylated at Thr41 (~ 94 kDa).

Immunogen

synthetic phosphopeptide corresponding to amino acids 38-50 (pThr41) of human β-catenin, conjugated to KLH.

Application

Monoclonal Anti-phospho-β-Catenin (pThr41) antibody produced in mouse may be used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunoblotting
  • immunoprecipitation

Biochem/physiol Actions

β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. β-Catenin activity is regulated by phosphorylation on serine/threonine residues located at the protein amino terminus.. The phosphorylation of β-catenin occurs in a protein complex that includes the APC complex (Adenomatous Polyposis Coli), together with Axin by casein kinase I (CKI) and glycogen synthase kinase-3 (GSK-3). The phosphorylation of the β-catenin protein targets the protein to degradation by the β-TrCP (β-transducin repeat-containing protein). Mutations in the serine and threonine residues in the amino-terminal region of the protein and in particular in the phosphorylation sites in that region, cause a reduction in the degradation of the protein and can lead to cancer. Monoclonal antibodies to phospho- β-catenin (pThr41) are an important tool for studying the regulation of β-catenin by phosphorylation and thus the transcription control of many proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wntbeta-catenin signaling in adult mammalian epithelial stem cells
Kretzschmar K and Clevers H
Developmental Biology, 428(2), 273-282 (2017)
Threonine 41 in beta-catenin serves as a key phosphorylation relay residue in beta-catenin degradation
Wu G and He, Xi X
Biochemistry, 45(16), 5319-5323 (2006)
Stéphanie Olivier-Van Stichelen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(8), 3325-3338 (2014-04-20)
Dysfunctions in Wnt signaling increase β-catenin stability and are associated with cancers, including colorectal cancer. In addition, β-catenin degradation is decreased by nutrient-dependent O-GlcNAcylation. Human colon tumors and colons from mice fed high-carbohydrate diets exhibited higher amounts of β-catenin and
Debasish Paul et al.
The Journal of pathology, 248(3), 266-279 (2019-02-05)
Aberrant activation of β-catenin has been implicated in a variety of human diseases, including cancer. In spite of significant progress, the regulation of active Wnt/β-catenin-signaling pathways is still poorly understood. In this study, we show that F-box protein 16 (FBXO16)
Molecular genetic analysis of malignant melanomas for aberrations of the WNT signalling pathway genes CTNNB1, APC, ICAT and BTRC
Reifenberger J, et al.
International Journal of Cancer. Journal International Du Cancer, 100(5), 549-556 (2002)

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