C8058
Chondroitinase B from Flavobacterium heparinum
lyophilized powder (with BSA as stabilizer)
Synonym(s):
Chondroitin sulfate B lyase
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About This Item
Biological source:
bacterial (Flavobacterium heparinum)
Pricing and availability is not currently available.
biological source
bacterial (Flavobacterium heparinum)
Quality Segment
conjugate
(Glucosaminoglycan)
form
lyophilized powder (with BSA as stabilizer)
shipped in
dry ice
storage temp.
−20°C
Application
Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.
The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide.[1] It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.[2]
Biochem/physiol Actions
Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co2+, Fe3+ and Ba2+ also inhibit its activity.[3]
Other Notes
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.
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This Item | |||
|---|---|---|---|
| biological source bacterial (Flavobacterium heparinum) | biological source - | biological source - | biological source - |
| form lyophilized powder (with BSA as stabilizer) | form lyophilized solid | form lyophilized powder | form lyophilized powder |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate (Glucosaminoglycan) | conjugate (Glucosaminoglycan) | conjugate (Glucosaminoglycan) | conjugate (Glucosaminoglycan) |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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