This item is tested in-house at the following conditions: T = 37 °C, pH = 5.0.
Other sources have found the optimum temperature range for this enzyme to be 40~60 °C.
C1184
Cellulase from Aspergillus niger
powder, ≥0.3 units/mg solid
Synonym(s):
1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
eCl@ss:
32160410
Specific activity:
≥0.3 units/mg solid
Pricing and availability is not currently available.
form
powder
Quality Segment
specific activity
≥0.3 units/mg solid
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
storage temp.
2-8°C
General description
The FDA recognizes cellulase from A. niger as GRAS (Generally Regarded As Safe) if non-pathogenic and non-toxigenic strains, as well as current good manufacturing practices, are used in production.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Cellulase belongs to the family of glycoside hydrolase, which is secreted by various cellulolytic microorganisms.
Cellulase belongs to the family of glycoside hydrolase, which is secreted by various cellulolytic microorganisms.
Application
The enzyme has also been approved as a secondary direct food additive as an aid in clam and shrimp processing.[1]
Cellulase from Sigma has been used to study the ability of several of its possible substrates, cellulose, Avicel PH-101, and filter paper, to protect enzyme activity during monogastric diegstion in animal and avian digestive tracts.
Biochem/physiol Actions
Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| specific activity ≥0.3 units/mg solid | specific activity ~0.8 U/mg | specific activity ≥1 unit/mg solid | specific activity ≥5,000 units/g solid |
| form powder | form powder | form lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| greener alternative category , Enabling | greener alternative category - | greener alternative category | greener alternative category |
| sustainability Greener Alternative Product | sustainability - | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
pcodes
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What is the optimum temperature of this enzyme?
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Can you please provide the percent purity of the product?
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The product is offered on the basis of activity. The purity has not been determined.
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Does product C1184 have hemicellulase activity, such as xylanase? I am looking for cellulase without hemicellulase activity.
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Hemicellulase activity of this product has not been tested.
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Are there any additives to Cellulase, Product C1184, in addition to the enzyme itself?
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Per information from our supplier, maltodextrin is an extender in this product. Maltodextrin can vary in glucose chain lengths of 2-12. There can also be some free glucose in the product. Either sorbitol or propylene glycol is added to the product as a stabilizer during processing. These components may be "flashed off" during processing, but some small amount may still be present.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product C1184, Cellulase from Aspergillus niger?
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The molecular weight is 26,000 Daltons.
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What is the solution stability of Cellulase, Product C1184?
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Sigma-Aldrich has not determined the solution stability for this product. However, it is reported in Methods in Enzymology, 160, 264 (1988) that cellulase from Aspergillus niger is "completely stable over the range of pH 5.0-8.0 at 4°C for 24 hours and retains about 50% of its original activity after heating at 70°C for 10 minutes. The enzyme is completely inactivated by heating at 80°C for 10 minutes".
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