For most applications, 10 to 800 nM works well (based on a molecular weight of 6512 g/mol). This information may be found in the technical bulletin for the Protease Inhibitor Panel, Product No. INHIB1.
A6103
Aprotinin
recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay
Synonym(s):
BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor
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Product Name
Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)
recombinant
expressed in Nicotiana (tobacco)
Quality Level
description
recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)
product line
BioUltra
Assay
≥98% (SDS-PAGE)
form
powder
specific activity
≥5 TIU/mg protein
mol wt
~_6.5 kDa
technique(s)
inhibition assay: suitable
solubility
0.85% sodium chloride: 5 mg/mL
UniProt accession no.
storage temp.
2-8°C
Gene Information
cow ... PTI(404172)
Unit Definition
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.
Preparation Note
Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
Protocols
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
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At what concentration should I use Product A6103, Aprotinin bovine?
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How does Product A6103, Aprotinin bovine, compare to Trasylol?
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Trasylol is the Bayer AG registered trademark name for this material.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you assay this the activity of Product A6103, Aprotinin bovine, in TIU or in KIU?
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We express the product's activity in TIU (Trypsin Inhibitor Units). Multiply the activity on the Certificate of Analysis by 1300 to convert it to KIU (Kallikrein Inhibitor Units).
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How should I store solutions of Product A6103, Aprotinin bovine?
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We generally advise storing stock solutions of approximately 5-10 mg/mL. Working solutions down to 0.065 micrograms/mL can be stored refrigerated at 2-8°C for one week, or at - 20°C for appoximately six months in single use aliquots. See Table 2 in the Datasheet for details.
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Product A6103, recombinant bovine aprotinin has a specification of ~60% protein. What is the remaining components of the solid?
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Product A6103, recombinant bovine aprotinin is >98% purity by SDS-PAGE. The product is ~60% protein with the balance being salts (NaCl).
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