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Sigma-Aldrich

Ovalbumin-Atto 488 conjugate

suitable for fluorescence

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 501 nm; λem 523 nm in PBS

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Fluorescent labeled ovalbumin is used as endocytic tracer.
Ovalbumin-Atto 488 conjugate may be used to monitor ovalbumin binding, transport and distribution in tissues. It may be used to study cell processes such as endocytosis.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Heli Jokela et al.
European journal of immunology, 50(10), 1500-1514 (2020-05-28)
Macrophages, which are highly diverse in different tissues, play a complex and vital role in tissue development, homeostasis, and inflammation. The origin and heterogeneity of tissue-resident monocytes and macrophages in ovaries remains unknown. Here we identify three tissue-resident monocyte populations
Achmet Imam Chasan et al.
Methods in molecular biology (Clifton, N.J.), 960, 379-388 (2013-01-19)
Isolation and characterization of antigen-containing endosomes remains difficult utilizing standard purification techniques. Here, we describe a method, which allows isolation of antigen-loaded endosomes, that is based on flow cytometrical analysis and sorting. We specifically isolated antigen-containing endosomes from cells that
Jet Phey Lim et al.
Biology open, 1(9), 904-914 (2012-12-06)
Macropinocytosis mediates the bulk endocytosis of solute molecules, nutrients and antigens. As this endocytic pathway is considered important in functions associated with immune responses, the molecular mechanisms regulating this pathway in immune cells is of particular significance. However, the regulators
Tamara Hilmenyuk et al.
Immunology, 129(3), 437-445 (2009-11-20)
Advanced glycation endproducts (AGEs) of food proteins resulting from the Maillard reaction after cooking or heating may have particular importance in food allergy. The underlying immunological mechanisms are only poorly understood. The aim of the study was to examine the
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