69198

Fraser Broth, Base

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Listeria Selective Enrichement Broth, Base (acc. Fraser)
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: casein enzymic hydrolysate, 5.0 g/L ; disodium phosphate dihydrate, 12.0 g/L ; esculin, 1.0 g/L ; lithium chloride, 3.0 g/L ; meat extract, 5.0 g/L ; peptic digest of animal tissue, 5.0 g/L ; potassium dihydrogen phosphate, 1.35 g/L ; sodium chloride, 20.0 g/L ; yeast extract, 5.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.2±0.2 (25 °C)
• application(s): agriculture; environmental; food and beverages; pathogen testing; sample preparation; microbiology
• suitability: selective and differential for Listeria spp.

Description

Application

Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds

Preparation Note

Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves