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Millipore

Enterococcus Selective Agar

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Slanetz and Bartley Agar, m-Enterococcus Agar acc. Slanetz and Bartley

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

Agency

according to ISO 7899-2:2000

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 10 g/L
disodium hydrogen phosphate monohydrate, 4 g/L
D(+)-glucose, 2 g/L
sodium azide, 0.4 g/L
2,3,5-triphenyltetrazolium chloride, 0.1 g/L
tryptose, 20 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

bioburden testing
clinical testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Enterococcus spp.
selective and differential for Streptococcus spp.

Related Categories

Application

Selective medium for enumeration and detection of enterococci in water and sewage with the membrane-filter technique and in the direct application procedure in food.

Preparation Note

Dissolve 42 g in 1 litre distilled water. Sterilize in a steamer without excess pressure for 30 minutes. Do NOT autoclave. Pour plates.

Other Notes

Isolation and enumeration of sulphite-reducing Clostridium spores from Group D faecal streptococci from oysters

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL5994
BCCK2937
BCCJ7292
BCCH6687
BCCG8467

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M K BURKWALL et al.
Applied microbiology, 12, 18-23 (1964-01-01)
A total of 15 agar media were examined for their yield, selectivity, readability, and simplicity of preparation and use. A thallium medium of Barnes was selected as the better of the high yield-fair selectivity type of medium and an azide-citrate
Xili Liang et al.
Applied microbiology and biotechnology, 102(11), 4951-4962 (2018-04-09)
Insects constitute the most abundant and diverse animal class and act as hosts to an extraordinary variety of symbiotic microorganisms. These microbes living inside the insects play critical roles in host biology and are also valuable bioresources. Enterococcus mundtii EMB156
Francois Lebreton et al.
Infection and immunity, 77(7), 2832-2839 (2009-05-13)
Enterococcus faecalis is an opportunistic pathogen that causes numerous infectious diseases in humans and is a major agent of nosocomial infections. In this work, we showed that the recently identified transcriptional regulator Ers (PrfA like), known to be involved in
Numbers of enterococci in water, sewage, and feces determined by the membrane filter technique with an improved medium.
L W SLANETZ et al.
Journal of bacteriology, 74(5), 591-595 (1957-11-01)
T J Easterbrook et al.
The Journal of applied bacteriology, 62(5), 413-419 (1987-05-01)
A comparative study of methods to enumerate sulphite-reducing Clostridium spores and Group D faecal streptococci in oysters demonstrated that pour plate solid agar techniques gave higher counts than liquid broth most probable number procedures. Reinforced clostridial broth with supplements to

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