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Key Documents

MABC1800

Sigma-Aldrich

Anti-EZHIP Antibody, clone 1G11

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G11, monoclonal

mol wt

calculated mol wt 51.89 kDa
observed mol wt ~60 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100 μg

technique(s)

immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

shipped in

2-8°C

target post-translational modification

unmodified

Gene Information

human ... EZHIP(340602)

General description

EZH inhibitory protein (UniProt: Q86X51; also known as EZHIP) is encoded by the EZHIP (also known as CXorf67) gene (Gene ID: 340602) in human. EZHIP is expressed predominantly in the gonads. In testis, it is detected in male germ cells inside the seminiferous tubules, especially in spermatogonia and round spermatids. In the ovary, it is expressed in primordial follicles and oocytes but not the external follicle cells. It is shown to inhibit PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation on lysine 27 (H3K27me3). It inhibits H3K27me3 deposition during spermatogenesis and oogenesis. Its inhibitory effect on methyltransferase activity is attributed to its ability to limit the stimulatory effect of cofactors such as AEBP2 and JARID2. Amino acid sequence 403-423 is reported to be essential and sufficient for inhibition of PRC2/EED-EZH1 and PRC2/EED-EZH2 complex activity and the sequence 401-409 is sufficient for its interaction with EZH2. (Ref.: Ragazzini, R., et al. (2019). Nat. Commun. 10(1); 3858; Jain, SU., et al. (2019). Nat. Commun. 10(1); Article 2146).

Specificity

Clone 1G11 is a mouse monoclonal antibody that detects EZH inhibitory protein. It targets an epitope within the N-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to 145 amino acids from the N-terminal half of human EZH inhibitory protein (EZHIP).

Application

Quality Control Testing

Evaluated by Western Blotting in Daoy cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EZHIP in Daoy cell lysates.

Tested Applications

Immunofluorescence Analysis: A 1:1500 dilution from a representative lot detected EZHIP in Daoy cells.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated EZHIP in Daoy cell lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-EZHIP, clone 1G11, Cat. No. MABC1800, is a mouse monoclonal antibody that detects EZH inhibitory protein (EZHIP) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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