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Key Documents

AB1834P

Sigma-Aldrich

Anti-Monocyte Chemotactic Protein-1 Antibody

Chemicon®, from rabbit

Synonym(s):

MCP-1, MCAF, CCL2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
neutralization: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CCL2(6347)

Specificity

Reacts with rat MCP-1

Immunogen

Highly pure (>98%) recombinant rat MCP-1

Application

Anti-Monocyte Chemotactic Protein-1 Antibody is an antibody against Monocyte Chemotactic Protein-1 for use in ELISA, WB, NEUT.
ELISA: To detect rat MCP-1 (MCAF) by direct ELISA (using 100 μL/well antibody solution) a concentration of at lease 0.5 μg/mL of this antibody is required. Should allow detection of 0.2 - 0.4 ng/well of recombinant rat MCP-1 (MCAF).

Western blot: 0.1 - 0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat MCP-1 (100 ng/mL), a concentration of 4.0-6.0 μg/mL of this antibody is required.

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors

Physical form

Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.4. Contains no preservative. Reconstitute with sterile distilled water to 0.1 - 1.0 mg/ml.

Storage and Stability

Maintain lyophilized material desiccated at 2°-8&deg:C for up to 12 months from date of receipt. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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