557440

Ru360

≥97%, solid, Ca2+ uptake blocker in mitrochondria, Calbiochem^®

• Synonym(s): Ru360, (μ)[(HCO₂)(NH₃)4Ru]₂OCl₃
• Empirical Formula (Hill Notation): C₂H₂₆Cl₃N₈O₅Ru₂
• Molecular Weight: 550.78
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥97%
• Form: solid
• Storage condition: OK to freeze, desiccated (hygroscopic), protect from light

Properties

• Product Name: Ru360, Ru360, is a cell-permeable oxygen-bridged dinuclear ruthenium amine complex. Binds to mitochondria with high affinity (K_d = 340 pM) and blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM).
• Quality Segment: 100
• assay: ≥97%
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic), protect from light
• color: green
• solubility: deoxygenated water: 0.5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A cell-permeable oxygen bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 0.34 nM) and specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (for complete block after incubation with ~10 µM of Ru360 for 30 min. Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction even at micromolar levels.

A cell-permeable oxygen-bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 340 pM). Specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (complete block after incubation with ~10 µM of Ru360 for 30 min). Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction, even at micromolar levels.

Note: 1 set = 10 x 100 µg.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Ca2+ uptake into mitochondria in vitro

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 184 pM blocking Ca2+ uptake into mitochondria in vitro; and in situ in intact myocytes; Kd = 340 pM for binding mitochondria

Packaging

Packaged under inert gas

Preparation Note

Unstable in solution; reconstitute just prior to use.

Other Notes

Sanchez, J.A., et al. 2001. J. Physiol. 536, 387.
Bassani, R.A., et al. 1998. Cell Calcium23, 433.
Matlib, M.A., et al. 1998. J. Biol. Chem.273, 10223.
Zhou, Z., et al. 1998. J. Physiol.507 (pt 2), 379.
Emerson, J., et al. 1993. J. Am. Chem. Soc.115, 11799.
Ying, W.-L., et al. 1991. Biochemistry30, 4949.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable