207008

Calcineurin Substrate

An excellent peptide substrate for calcineurin.

• Synonym(s): Calcineurin Substrate, PP2B Substrate, RII Phosphopeptide, DLDVPIPGRFDRRVpSVAAE
• Empirical Formula (Hill Notation): C₉₂H₁₅₁N₂₈O₃₂P
• Molecular Weight: 2192.33
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: water: 2 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

An excellent peptide substrate for calcineurin (Protein Phosphatase 2B, PP2B, Cat. No. 539568). The sequence is derived from the PKA regulatory subunit type II (RII) and is phosphorylated on the Ser residue. Release of phosphate can be quantified colorimetrically using malachite green method.

An excellent peptide substrate for calcineurin (Protein Phosphatase 2B, PP2B; Cat. No. 539568). The sequence is derived from the PKA regulatory subunit type II (RII) and is phosphorylated on the Ser residue. Release of phosphate can be quantified colorimetrically using the malachite green method.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Calcineurin (Protein Phosphatase 2B, PP2B)

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
Enz, A., et al. 1994. Anal. Biochem. 216, 147.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Fruman, D.A., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 3686.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable