1.04172

Toluidine blue O (C.I.52040)

• Empirical Formula (Hill Notation): C₁₅H₁₆ClN₃S
• CAS Number: 92-31-9
• Molecular Weight: 305.83
• MDL number: MFCD00011934
• UNSPSC Code: 41116130
• NACRES: NA.21

Properties

• form: solid ()
• Quality Level: 200
• composition: Dye, ≥85% spectrophotometric assay
• pH: 2.8 (25 °C in H₂O)
• solubility: 30 g/L
• bulk density: 410 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C15H16N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H/q+1;/p-1
• InChI key: HNONEKILPDHFOL-UHFFFAOYSA-M

Description

General description

Toluidine blue O (C.I. 52040) for microscopy Certistain^® is adry dye that is used for the preparation of a staining solution for staining nuclei and for visualization of metachromasia. As a CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain^®

Analysis Note

Dye content (Diaminophenothiazins, spectrophotometrically): ≥ 85 %
Identity
- chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 628 - 633 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; water): 1330 - 1570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable