Saltar al contenido
Merck

RP-HPLC Analysis of Benzodiazepines on Ascentis® Express Biphenyl

RP-HPLC Analysis of Benzodiazepines on Ascentis® Express Biphenyl application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 3.0 mm

standard

Referencia del producto
Descripción
Precios

Oxazepam solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Alprazolam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Clonazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Diazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Flunitrazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Lorazepam solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Nitrazepam solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Temazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (64066-U)

mobile phase

[A] Water; [B] acetonitrile; (70:30, A:B)

flow rate

1.0 mL/min

pressure

5294 psi (365 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

1.0 μL

sample

Benzodiazepine Multicomponent Mixture-8 diluted to 50ug/mL in 50:50 Water:Methanol

Descripción

Analysis Note

This analysis demonstrates the applicability of the Ascentis Express Biphenyl stationary phase for the analysis of benzodiazepines, a group of psychoactive drugs that act as GABA agonists. The stationary phase provides alternative selectivity and improved resolution to existing phenyl chemistires.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany