HPLC Analysis of Tryptic Digest on BIOshell A160 Peptide C18
Materiales
Analytical column
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Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 15 cm × 2.1 mmStandard
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Casein from bovine milk
suitable for substrate for protein kinase (after dephosphorylation), purified powderCONDITIONS
column
BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)
mobile phase
[A] 0.1% formic acid in water; [B] 25:75, (0.4% formic acid in water):acetonitrile
gradient
0 to 70% B in 40 min.
flow rate
0.3 mL/min
column temp.
35 °C
detector
ESI(+), TOF
injection
2 μL
sample
10 pg/L in 0.1% formic acid
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