HPLC Analysis of Protein Standards on Zenix^® SEC-300 using Different Buffer Systems

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)

mobile phase

[A] 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid; [B] 0.1 M ammonium bicarbonate (pH unadjusted)

flow rate

0.3 mL/min

column temp.

25 °C

detector

UV, 280 nm

detector

1000 psi

injection

2.5 μL

sample

in mobile phase