HPLC Analysis of Norphenylephrine Enantiomers on Astec® CYCLOBOND I 2000 AC (pH 5.0)
CONDITIONS
column
CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)
mobile phase
[A] methanol; [B] 20 mM ammonium acetate, pH 5.0; (5:95, A:B)
flow rate
0.5 mL/min
column temp.
25 °C
detector
UV, 230 nm
injection
5 μL
sample
norphenylephrine, 1 mg/mL in acetonitrile:water (50:50)
Descripción
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
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