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HPLC Analysis of Glutethimide Enantiomers on Astec® Cellulose DMP

HPLC Analysis of Glutethimide Enantiomers on Astec® Cellulose DMP application for HPLC

Materiales

analytical column

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Astec® Cellulose DMP Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

mobile phase component

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2-Propanol

99.5%, HPLC grade

standard

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DL-Glutethimide

CONDITIONS

column

Astec Cellulose DMP, 25 cm x 4.6 mm I.D., 5 μm particles (51099AST)

mobile phase

[A] heptane; [B] isopropanol; (80:20, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

15 μL

sample

glutethimide, 1 g/L in heptane:isopropanol (50:50)

Descripción

Analysis Note

Glutethimide enantiomers are resolved by normal phase chromatography.

Other Notes

Glutethimide is used to treat insomnia.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany