HPLC Analysis of Atrazine Herbicides on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] 20 mM ammonium acetate, pH 6.4 unadjusted; [B] acetonitrile

gradient

20 to 28% B in 11 min; 28 to 65% B in 5 min; held at 65% B for 4 min

flow rate

0.6 mL/min

column temp.

46 °C

detector

UV, 240 nm

injection

5 μL

sample

10-25 μg/mL in 10:90, water: acetonitrile