Fatty acids, free

column

Nukol, 15 m × 0.53 mm I.D., 0.5 μm (25326)

oven

110 °C, 8 °C/min to 220 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

helium, 20 mL/min

injection

1 μL, direct

sample

25-400 μg/mL each analyte