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Merck

Y1250

Sigma-Aldrich

Yeast Nitrogen Base With Amino Acids

Non-selective yeast growth medium

Sinónimos:

ynb with amino acids

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250 G
US$ 660,00

US$ 660,00

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Disponible para envío el07 de abril de 2025Detalles


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250 G
US$ 660,00

About This Item

Código UNSPSC:
41106212
NACRES:
NA.85

US$ 660,00

PRECIOS SIN IMPUESTOS NACIONALES

Disponible para envío el07 de abril de 2025Detalles


Solicitar un pedido a granel

grado

Molecular Biology
for molecular biology

esterilidad

non-sterile

Formulario

powder

técnicas

microbiological culture: suitable

pH

5.3-5.5(0.67% solution)

aplicaciones

microbiology

temp. de almacenamiento

room temp

idoneidad

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Descripción general

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.

Aplicación

Yeast Nitrogen Base With Amino Acids has been used:
  • in the ZYP-31 media preparation for E.coli[1]
  • in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source[2]
  • to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard[3]
  • in the preparation of imaging medium[4]

Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.

Componentes

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Reconstitución

Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Visite la Librería de documentos

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Ude C, et al.
Sensors and Actuators B, Chemical, 221, 1035-1043 (2015)
G U Balcke et al.
Toxicology letters, 203(3), 200-209 (2011-03-16)
The study presented here describes the application of metabolite profiling of highly polar, intracellular metabolites after incubation of a mammalian fibroblast cell line with inhibitors of mitochondrial function. A metabolomics approach was used to assess the complex response of the
Corinne Maufrais et al.
G3 (Bethesda, Md.) (2021-04-07)
The genomes of a large number of Cryptococcus neoformans isolates have been sequenced and analyzed in recent years. These genomes have been used to understand the global population structure of this opportunistic pathogen. However, only a small number of South
The role of Aspartyl aminopeptidase (Ape4) in Cryptococcus neoformans virulence and authophagy
de AGF, et al.
PLoS ONE, 12(5), e0177461-e0177461 (2017)
Live bio-imaging with fully bio-compatible organic fluorophores
Yang I, et al.
Journal of Photochemistry and Photobiology. B, Biology, 166, 52-57 (2017)

Protocolos

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Questions

1–3 of 3 Questions  
  1. Is the Yeast nitrogen base with amino acids food grade or FCC

    1 answer
    1. This item is intended for research use only and is not suitable as food grade or FCC material. Currently, there is no product option available that meets this requirement.

      Helpful?

  2. Is there a technical data sheet avaliable for product Y1250? I would like to know the concentrations of trace elements.

    1 answer
    1. The concentration of the trace elements is not reported on the datasheet. The trace elements in the formulation are as follows:

      - Boric Acid: 500.0µg
      - Potassium Iodide: 100.0µg
      - Manganese Sulfate: 400.0µg
      - Zinc Sulfate: 0.4mg
      - Calcium Chloride: 0.1g
      - Copper Sulfate: 40.0µg
      - Ferric Chloride: 200.0µg

      Helpful?

  3. Hi, Is this product Kosher and Halal certified? And food grade? Y1250 - Sigma-Aldrich Yeast Nitrogen Base With Amino Acids Kr, Benedicte

    1 answer
    1. This product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals. Currently, a food-grade product is not offered.

      Helpful?

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