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T1892

Sigma-Aldrich

Thioflavine S

Practical grade, powder

Sinónimos:

Direct Yellow 7

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25 G
US$ 145,00

US$ 145,00

Disponible para envío el01 de abril de 2025Detalles

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25 G
US$ 145,00

About This Item

Número de CAS:
1326-12-1
Número de índice de color:
49010
Número CE:
215-418-0
Número MDL:
MFCD00071453
Código UNSPSC:
12171500
NACRES:
NA.47

US$ 145,00

Disponible para envío el01 de abril de 2025Detalles

Solicitar un pedido a granel

Nombre del producto

Thioflavine S, practical grade

grado

practical grade

Formulario

powder

técnicas

microbe id | staining: suitable

color

light beige to dark beige, to Dark Brown

solubilidad

ethanol: water (1:1): 1 mg/mL

λmáx.

360-380

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

S1c2c(ccc(c2)C)N(C1=C3C=CC(=[N+](C)C)C=C3)C.[Cl-]

InChI

1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1

Clave InChI

JADVWWSKYZXRGX-UHFFFAOYSA-M

Descripción general

Thioflavine S aids in staining and visualizing β-amyloid plaques in Alzheimer′s disease. Thioflavin S binds to fibrillar β-amyloid plaques,[1][2] and does not diffuse in the plaques.[1] Thioflavin-S staining aids in visualizing smaller plaques throughout the neocortex, hippocampus and thalamic region.[1]

Aplicación

Thioflavine S has been used to stain and visualize β-amyloid structures in the tissue sections.[1][3][4][2]

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H315,H319,H335

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SHBS5830
SHBR0688
SHBP5084
SHBM5906
MKCH4108

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Visite la Librería de documentos

MRI and histological analysis of beta-amyloid plaques in both human Alzheimer's disease and APP/PS1 transgenic mice
Meadowcroft MD, et al.
Journal of Magnetic Resonance Imaging : JMRI, 29(5), 997-1007 (2009)
Genetic modulation of soluble A beta rescues cognitive and synaptic impairment in a mouse model of Alzheimer's disease
Fowler SW, et al.
The Journal of Neuroscience, 34(23), 7871-7885 (2014)
Disha Shah et al.
PloS one, 8(12), e84241-e84241 (2013-12-21)
Functional connectivity (FC) studies have gained immense popularity in the evaluation of several neurological disorders, such as Alzheimer's disease (AD). AD is a complex disorder, characterised by several pathological features. The problem with FC studies in patients is that it
Attenuation of beta-amyloid deposition and neurotoxicity by chemogenetic modulation of neural activity
Yuan P and Grutzendler J
The Journal of Neuroscience, 36(2), 632-641 (2016)
Exacerbation of cerebral amyloid angiopathy-associated microhemorrhage in amyloid precursor protein transgenic mice by immunotherapy is dependent on antibody recognition of deposited forms of amyloid beta
Racke MM, et al.
The Journal of Neuroscience, 25(3), 629-636 (2005)
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Questions

1–4 of 4 Questions  

  1. Thioflavine S requires any special microscope filter?

    1 answer

    1. Yes. Thioflavine S is a fluorescent dye. Excitation is at 391 nm and emission is at 428 nm. The microscope companies that sell fluorescent microscopes will be able to offer the suggested filter sets for the fluorescent microscope you are using

      This product is listed as a practical grade dye and is not highly purified. Quality control testing does not include testing for either the excitation or emission wavelengths.

      Helpful?

  2. How should Thioflavine S be reconstituted at 1mg/mL?

    1 answer

    1. For usage, Thioflavine S is typically dissolved at a concentration of 1 gram of dye in 100 ml of water (10mg/ml). Sections are taken to water and then stained for 7 minutes in the Thioflavine S solution. Slides are rinsed in 3 changes of 80% alcohol. Subsequently, the slides are dehydrated, cleared, and mounted in fluorescent free mounting media, such as DPX. Finally, the slides are viewed with a fluorescent microscope.

      Helpful?

  3. Regarding item T1892-25G, how much time is needed to rinse the section in 80% EtOH and what is the time for the dehydration step?

    1 answer

    1. When the time for rinse questions arises, it generally means the slides are rinsed in a particular solvent after the slides have been stained in the staining reagent. Protocols can and will vary for Thioflavine T. The procedure calls for deparaffinizing sections in the normal fashion. Slides are then stained in Mayers Hematoxylin for 2 minutes and washed in tap water for 3 to 5 minutes to blue the nuclei. Subsequently, the slides are stained for 3 minutes in the Thioflavine T solution. After this, the slides are rinsed in distilled water with no specified length of time. The slides are then differentiated in 1% acetic acid for 20 minutes, followed by a wash in running tap water for 2 minutes. Finally, the slides are blotted dry and mounted in an appropriate fluorescent mounting media. Other than the deparaffinization procedure, the slides are not rinsed in 80% alcohol. After the slides are blotted dry, there is no normal dehydration routine that is common to slides being mounted in a permanent mounting medium.

      Helpful?

  4. Whether Thioflavine S requires a conjugated secondary antibody or if it is the only stain to be applied to the tissue?

    1 answer

    1. No, Thioflavine S is a fluorescent dye that binds to amyloid and fibrillar material in abnormal structures. Sections requiring viewing under a fluorescence microscope with filters for Thioflavine S. Slides are typically stained for 7 minutes in a 1% solution of Thioflavine S prepared in distilled water. The slides are then washed in 3 changes of 80% alcohol. Subsequently, the slides are further dehydrated, cleared, and then mounted using a fluorescent-free mounting media. After these procedures, the slides are ready for viewing with a fluorescent microscope.

      Helpful?

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