SRP6433

PD-L1/CD274/B7-H1 from mouse

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinónimos: PDCD1L1, PDCD1LG1
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: mouse
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: lyophilized
• mol peso: calculated mol wt 25.6 kDa; observed mol wt 40-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Phe 19 is the predicted N-terminal.)
• envase: pkg of 10 and 50 μg
• impurezas: <1 EU/μg (LAL test)
• Nº de acceso UniProt: Q9EP73
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: mouse ... CD274(60533)

Descripción

Descripción general

Programmed cell death 1 ligand 1 (PD-L1), which is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules. PD-L1 is a cell surface immunoglobulin with two Ig-like domains within the extracellular region and a short cytoplasmic domain. The protein is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes.[1][2][3]

Acciones bioquímicas o fisiológicas

Programmed cell death 1 ligand 1 (PD-L1) is involved in the regulation of cellular and humoral immune responses. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after lipopolysaccharide (LPS) and interferon-γ (IFNG) activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the co-stimulatory signal, essential for T-cell proliferation and production of interleukin-10 (IL10) and IFNG, in an interleukin-2-dependent and a programmed cell death 1 (PDCD1)-independent manner.[4]

Forma física

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable