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SRP2106

Sigma-Aldrich

TATA box binding protein,GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Sinónimos:

GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~64.4 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

350 μg/mL

color

clear colorless

NCBI accession no.

NM_003194

UniProt accession no.

P20226

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TBP(6908)

Biochem/physiol Actions

The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to the TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

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Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
A Hoffmann et al.
Nucleic acids research, 19(22), 6337-6338 (1991-11-25)
M Horikoshi et al.
Cell, 54(7), 1033-1042 (1988-09-23)
The mammalian activator protein ATF stimulates transcription from the adenovirus E4 promoter by binding to multiple upstream promoter and enhancer elements. DNAase footprint analyses have revealed that there are cooperative interactions between ATF and TFIID (the mammalian TATA factor) when
TBP, a universal eukaryotic transcription factor?
N Hernandez
Genes & development, 7(7B), 1291-1308 (1993-07-01)

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