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SRP0255

Sigma-Aldrich

eIF4E Active human

N-terminal GST-tag, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Sinónimos:

CBP, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

human

recombinante

expressed in E. coli

Ensayo

≥70% (SDS-PAGE)

Formulario

aqueous solution

mol peso

51 kDa

envase

pkg of 100 μg

concentración

>0.02 mg/mL

técnicas

cell based assay: suitable

idoneidad

suitable for molecular biology

Nº de acceso NCBI

NM_001968

Nº de acceso UniProt

P06730

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... EIF4E(1977)

Descripción general

EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein.[1] It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.[2][3]

Aplicación

Suitable substrate for MNK1 kinase.

Acciones bioquímicas o fisiológicas

EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.[1][4]

Forma física

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
40530100901
4053000901

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Eukaryotic Translation Initiation Factor 4E Is a Feed-Forward Translational Coactivator of Transforming Growth Factor ? Early Protransforming Events in Breast Epithelial Cells.
Decarlo L, et al.
Molecular and Cellular Biology, 35(15), 2597-2609 (2015)
Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E.
Pyronnet S, et al.
The Embo Journal, 18(1), 270-279 (1999)
Eukaryotic translation initiation factor 4E (eIF4E) binding site and the middle one-third of eIF4GI constitute the core domain for cap-dependent translation, and the C-terminal one-third functions as a modulatory region.
Morino S, et al.
Molecular and Cellular Biology, 20(2), 468-477 (2000)
Yao Zhan et al.
The Journal of investigative dermatology, 135(5), 1368-1376 (2015-01-24)
In eukaryotic cells, the rate-limiting component for cap-dependent mRNA translation is the translation initiation factor eIF4E. eIF4E is overexpressed in a variety of human malignancies, but whether it has a role in melanoma remains obscure. We hypothesized that eIF4E promotes

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