SRE0023

β-Glucuronidase from abalone

Purified, aqueous solution, β-glucuronidase 150,000-250,000 units/mL, β-glucuronidase ≥20,000,000 units/g protein

• Comisión internacional de enzimas: 3.1.2.31 (BRENDA, IUBMB)
• Número MDL: MFCD00130629
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• Nivel de calidad: 200
• Formulario: aqueous solution
• β-glucuronidase activity: ≥20,000,000 units/g protein; 150,000-250,000 units/mL
• secondary activity: ≤7,500 units/mL sulfatase
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

Acciones bioquímicas o fisiológicas

β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans.[1] β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations.[2] Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.[1]

Definición de unidad

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Forma física

Supplied as an aqueous solution containing L-proline, ammonium acetate and sodium azide

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable