SAB4200522

Anti-IDH1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Sinónimos: Anti-IDP, Anti-PICD, Anti-isocitrate dehydrogenase 1 (NADP+), soluble, IDH
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~45 kDa
• reactividad de especies: rat, mouse, human
• concentración: ~1.0 mg/mL
• técnicas: immunohistochemistry: 20 μg/mL using human kidney.; indirect immunofluorescence: 1-2 μg/mL using SHSY5Y cells.; western blot: 1-2 μg/mL using extracts of NIH3T3 cells and of rat cerebellum (S1 fraction).
• Nº de acceso UniProt: O75874
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... IDH1(3417) ; mouse ... Idh1(15926) ; rat ... Idh1(24479)

Descripción

Descripción general

IDH1 is a cytoplasmic nicotinamide adenine dinucleotide phosphate (NADP⁺)-dependent enzyme, localized both in the cytoplasm and peroxisomes. The IDH1 gene is mapped to human chromosome 2q34. It comprises a tripeptide peroxisome targeting signal 1 in the C-terminal region and is majorly expressed in the liver.

Especificidad

Anti-IDH1 (C-terminal)specifically recognizes human, rat and mouse IDH1.

Inmunógeno

synthetic peptide corresponding to a C-terminal sequence of human IDH1, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse IDH1.

Aplicación

Anti-IDH1 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Acciones bioquímicas o fisiológicas

Isocitrate dehydrogenase (IDH) is a key metabolic enzyme that catalyzes the oxidative decarboxylation of isocitrate into α-ketoglutarate (αKG) utilizing either nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺) as co-substrates. Although the function and structure of IDH1 have been well characterized, it has only recently been implicated in cancer. IDH1 appears to have a tumor suppressor activity and its inactivation contributes to tumorigenesis partially mediated by induction of the hypoxia-inducible factor 1α (HIF1) pathway. A genome-wide mutation study has shown that IDH1 is mutated in glioblastoma, acute myeloid leukemia (AML) and chondrosarcoma. Mutations in IDH1 specific to Arg132 (R132) impart the enzyme the ability to generate 2-hydroxyglutarate (2HG) instead of αKG. Elevated levels of 2HG are correlated with increased risk of malignant brain tumors, and block cell differentiation by impairing histone demethylation. Several IDH1 mutations have been identified in gliomas, including R132H, R132C, R132S, R132G and R132L, each may result in different tumor type with varied malignant progression.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, storeat 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable