SAB4200477

Anti-ALIX (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Sinónimos: Anti-PDCD6IP, programmed cell death 6 interacting protein, AIP1, DRIP4, HP95, ALG-2 interacting protein 1, Anti-apoptosis-linked gene 2-interacting protein X, Anti-dopamine receptor interacting protein 4
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~95 kDa
• reactividad de especies: human, mouse
• concentración: ~1.0 mg/mL
• técnicas: immunoprecipitation (IP): 1-2 μg using lysates of human Jurkat cells; indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells; western blot: 1-2 μg/mL using whole extracts of mouse A20 cells
• Nº de acceso UniProt: Q8WUM4
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PDCD6IP(10015) ; mouse ... Pdcd6ip(18571)

Descripción

Descripción general

The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region.

Especificidad

Anti-ALIX (C-terminal) recognizes human and mouse ALIX.

Inmunógeno

peptide corresponding to a sequence at the C-terminal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in mouse, pig, monkey, bovine and dog and differs by a single amino acid in rat.

Aplicación

Anti-ALIX (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting[1]
• immunoprecipitation
• immunofluorescence

Acciones bioquímicas o fisiológicas

Apoptosis-linked gene 2-interacting protein X (ALIX) protein binds to endophilin proteins that regulate membrane shape during endocytosis. Over-expression of the ALIX gene and endophilin leads to cytoplasmic vacuolization, which may be a reason for protection against cell death.

PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery.

Forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable