R7260
Xba I from Xanthomonas badrii
Restriction Enzyme
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352204
Productos recomendados
![[4-(acetylamino)phenyl]acetic acid AldrichCPR](/deepweb/assets/sigmaaldrich/product/structures/998/245/c05afb37-e406-430e-b87e-95b0335140f4/640/c05afb37-e406-430e-b87e-95b0335140f4.png)
grade
for molecular biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Specificity
Recognition sequence: 5′-T/CTAGA-3′
Cutting results: A 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
Cutting results: A 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
Application
XbaI is a restriction enxyme that cleaves DNA at the recognition site 5′-T/CTAGA-3′ to generate DNA fragments with 5′-cohesive termini.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Xba I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains). May cleave at secondary recognition sites under non-optimal conditions.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 0.1 mM PMSF, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.02% polydocanol at 4 °C
related product
Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Stephanie L H Miller et al.
The Journal of biological chemistry, 279(32), 33528-33537 (2004-05-25)
The ubiquitin-interacting motif (UIM) is a short peptide motif with the dual function of binding ubiquitin and promoting ubiquitylation. This motif is conserved throughout eukaryotes and is present in numerous proteins involved in a wide variety of cellular processes including
A new specific endonuclease from Xanthomonas badrii..
Zain, B.S., and Roberts, R.J.,
Journal of Molecular Biology, 115, 244-255 (1977)
Miyuki Watabe et al.
The Ulster medical journal, 77(3), 168-174 (2008-10-30)
In Northern Ireland over the last 7 years, there is a mean of 41.9 laboratory reports per annum of human gastrointestinal infection (range 19-54) caused by Escherichia coli O157:H7. In the preceding years 1992-1996, reports were 5.4 per annum, whereas
E Malyguine et al.
Gene, 8(2), 163-177 (1980-01-01)
The specificity of XbaI, SalI, HhaI, PstI, BamHI and SstI is relaxed in the presence of an organic solvent, such as 20% glycerol or 8% dimethylsulfoxide (DMSO). This alteration, very pronounced in some cases, requires an excess of enzyme, varies
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico