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MAK190

Sigma-Aldrich

ATP Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Sinónimos:

Adenosine triphosphate Assay Kit

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About This Item

Número CE:
213-579-1
Código UNSPSC:
12161503
NACRES:
NA.84

método de detección

colorimetric
fluorometric

enfermedades relevantes

cancer; neurological disorders

temp. de almacenamiento

−20°C

Descripción general

Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes.[1][2] Genetic disorders such as Leber hereditary optic neuropathy,[2] Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa [3] affect the generation of ATP in the mitochondria.

Aplicación

ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.[4]

Idoneidad

Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.

Principio

The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.

Otras notas

The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.

Solo componentes del kit

Referencia del producto
Descripción
  • ATP Assay Buffer
  • ATP Probe, in DMSO
  • ATP Converter
  • Developer Mix
  • ATP Standard, 1.0 μmole

sustituido por

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F

Punto de inflamabilidad (°C)

87 °C

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Certificados de análisis (COA)

Lot/Batch Number
8L09K03540
8E04K03540
8A07K03540
7H23K03540
7D05K03540

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Cost of surviving sepsis: A novel model of recovery from sepsis in Drosophila melanogaster.
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Intensive care medicine experimental, 4(1), 4-4 (2016)
Rosalba Carrozzo et al.
Bioscience reports, 27(1-3), 105-112 (2007-05-09)
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Melatonin Balance the Autophagy and Apoptosis by Regulating UCP2 in the LPS-Induced Cardiomyopathy.
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Mitochondria are key players in several cellular functions including growth, division, energy metabolism, and apoptosis. The mitochondrial network undergoes constant remodelling and these morphological changes are of direct relevance for the role of this organelle in cell physiology. Mitochondrial dysfunction
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Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the chemical composition of the reagents?

    1 answer

    1. The chemical composition of these reagents is proprietary.

      Helpful?

  4. Dear sir, We are interested in quantification of ATP levels "ATP Assay Kit" Cat.No:MAK190. Protocol recommends that cells should be deproteinized using 10kDa MWCO spin filter, but no protocol is mentioned. Can you Please send us protocol for the same.

    1 answer

    1. The Datasheet can be accessed in the DOCUMENTATION section under 'More Documents'. Please see the Datasheet below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

      Helpful?

  5. What is the chemical composition of the reagents?

    1 answer

    1. The chemical composition of these reagents are proprietary. Please see the link below to review the Product datasheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

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