Please see the link below to review the product technical bulletin. This document contains the preparation, dilution, and working concentrations of the reagents used: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/399/flascbul.pdf
FLASC
Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit
for cellular ATP determination
Sinónimos:
ATP bioluminescence assay kit
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FLASC-1KT
US$ 722,00
US$ 722,00
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UNSPSC Code:
12352202
NACRES:
NA.26
eCl@ss:
32160414
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storage temp.
−20°C
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General description
Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit may be employed for the bioluminescent determination of adenosine 5′-triphosphate (ATP) released from a suspension of viable somatic cells. Estimates of cell concentrations may be calculated if it is assumed that the ATP content per viable cell remains constant. The number of viable somatic cells are selectively counted because, as a cell dies, its ATP is rapidly degraded.
Application
The Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit has been used to determine accurately the level of ATP present in neutrophils isolated from the blood of over 100 human test patients. [1]
Solo componentes del kit
Referencia del producto
Descripción
- FL-AAB Dilution buffer 1 mL/vial
- FL-AAM Assay mix 1 mL/vial
- FL-AAS ATP standard 1 mL/vial
- FL-SAR Somatic cell releasing reagent 1 mL/vial
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Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
wgk
WGK 3
Clase de almacenamiento
11 - Combustible Solids
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Sarah Myhill et al.
International journal of clinical and experimental medicine, 2(1), 1-16 (2009-05-14)
This study aims to improve the health of patients suffering from chronic fatigue syndrome (CFS) by interventions based on the biochemistry of the illness, specifically the function of mitochondria in producing ATP (adenosine triphosphate), the energy currency for all body
Linqing Zhen et al.
Reproductive toxicology (Elmsford, N.Y.), 59, 66-79 (2015-11-20)
Hexavalent chromium reportedly induces reproductive toxicity and further inhibits male fertility in mammals. In this study, we investigated the molecular mechanism by which hexavalent chromium affects motility signaling in boar spermatozoa in vitro. The results indicated that Cr(VI) decreased sperm
Y Zhao et al.
Redox biology, 12, 129-138 (2017-02-24)
The signaling of reactive oxygen species (ROS) is essential for the maintenance of normal cellular function. However, whether and how ROS regulate stem cells are unclear. Here, we demonstrate that, in transgenic mice expressing the human manganese superoxide dismutase (MnSOD)
Lirui Wang et al.
Reproductive toxicology (Elmsford, N.Y.), 75, 23-32 (2017-11-22)
Cadmium (Cd) has been reported to inhibit mouse sperm motility by inducing the tyrosine phosphorylation of dihydrolipoamide dehydrogenase (DLD). This study aimed to assess the potential effects of vitamin C (Vc) on ameliorating Cd-induced tyrosine phosphorylation of DLD and the
Qicheng Ni et al.
Nature communications, 8, 15755-15755 (2017-06-10)
Diabetes is associated with beta cell mass loss and islet dysfunctions. mTORC1 regulates beta cell survival, proliferation and function in physiological and pathological conditions, such as pregnancy and pancreatectomy. Here we show that deletion of Raptor, which is an essential
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Instructions
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Is any protocol available for this product? What is the working concentration of each solution? Which reagent should be used to prepare dilutions?
1 answer-
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Can lysates be stored in the Somatic Cell ATP Releasing Reagent of Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?
1 answer-
In general, no. The working Somatic Cell ATP Releasing Reagent is intended for immediate use, and not for storing ATP in. We have no specific information as to how long ATP will be stable in it.
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Can Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC, be used for adherent cells?
1 answer-
We do not have a separate protocol for adherent cells. However, the somatic cell lysis reagent should be able to lyse cells that are adherent. The reagent contains enough detergent that the cells will be lysed. Once cells are lysed, it is best to assay the sample immediately.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the optimal dilution for tissue extracts when using Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?
1 answer-
Sigma-Aldrich has not tested this product with tissue extracts. You will need to perform trials to determine optimal dilution of the sample.
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