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MAK094

Sigma-Aldrich

Protein Carbonyl Content Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detección

colorimetric

enfermedades relevantes

cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases

temp. de almacenamiento

2-8°C

Descripción general

Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.

Aplicación

Protein carbonyl content assay kit has been used to determine protein carbonyl concentration.[1][2][3][4][5]

Idoneidad

Suitable for quantifying carbonyls in protein samples

Principio

The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.

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Referencia del producto
Descripción
Precios

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

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Certificados de análisis (COA)

Lot/Batch Number
8M13K08300
8F16K08300
8B07K08300
7L05K08300
7E05K08300

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Effects of chilled-then-frozen storage (up to 52 weeks) on an indicator of protein oxidation and indices of protein degradation in lamb M. longissimus lumborum.
Coombs CE, et al.
Meat Science, 135, 134-141 (2018)
Melissa M Miranda-Cruz et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 213, 19-26 (2018-07-25)
Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism
Benjamin W B Holman et al.
Meat science, 139, 171-178 (2018-02-11)
Different chilled (~0.1 °C for up to 5 weeks) then frozen storage (up to 12 months) combinations and two frozen storage holding temperatures (-12 °C and -18 °C) effects on beef M. longissimus lumborum (LL) protein structure degradation and a marker of protein oxidation were
Yujie Huang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(2), 758-779 (2018-08-31)
Skeletal muscle atrophy is an important health issue and can impose tremendous economic burdens on healthcare systems. Glucocorticoids (GCs) are well-known factors that result in muscle atrophy observed in numerous pathological conditions. Therefore, the development of effective and safe therapeutic
UV?B radiation interacts with temperature to determine animal performance.
Ghanizadeh-Kazerouni E, et al.
Functional Ecology, 30(4), 584-595 (2016)
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Could you please provide information on the type of buffer to use for isolating proteins from insect tissue in a way that will preserve the carbonylation for the MAK094-1KT Protein Carbonyl Content Assay Kit? Additionally, do you anticipate any adverse effects from Decolor paint pens on the cuticle of the insect tissue sample?

    1 answer

    1. It is recommended to homogenize cells using mechanical cell disruption in water only, as common buffer components can interfere with the protein carbonyl content of the sample. If the protein is very dilute, it can be concentrated using a 10 kDa spin filter. For the assay, it is advised to use 100 µL of sample containing approximately 0.5-2 mg protein and include a reagent background control by using 100 µL of dH2O alone. Regarding the impact of xylenes on this assay, it would need to be confirmed empirically.

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