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MAK074

Sigma-Aldrich

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

Vitamin C Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

método de detección

colorimetric
fluorometric

temp. de almacenamiento

−20°C

Descripción general

The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Idoneidad

Suitable for use with biological fluids, food samples, and growth media. Proteins present in the sample can interfere with this assay. Protein containing samples are best analyzed using the Ascorbic Acid Assay II (MAK075). Alternatively, samples can be deproteinized using a 10 kDa Molecular Weight Cut-Off Spin Filter.

Principio

In this assay, Ascorbic Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/ λem = 587 nm) product, proportional to the ascorbic acid present.

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Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H317,H319,H334

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
8L15K06610
7F22K06610
6B18K06610
6G20K06610
6A30K06610

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Giulia Costanzo et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-06-18)
Peel, pulp and seed extracts of three mandarin varieties, namely Phlegraean mandarin (Citrus reticulata), Kumquat (Citrus japonica), and Clementine (Citrus clementina) were compared and characterised in terms of photosynthetic pigment content, total polyphenols amount, antioxidant activity and vitamin C to
Ermenegilda Vitale et al.
Plants (Basel, Switzerland), 10(5) (2021-05-01)
This paper evaluates the combined effect of biostimulant and light quality on bioactive compound production and seedling growth of soybean (Glycine max L. Merrill) plants. Germinated seeds pre-treated with different concentrations (0.01%, 0.05%, 0.5%) of an amino acid-based biostimulant were
Giulia Costanzo et al.
Antioxidants (Basel, Switzerland), 11(2) (2022-02-26)
In this work, we assess the potential of waste products of Phlegrean mandarin (Citrus reticulata Blanco), namely seeds and peel, to be reutilized as a source of bioactive compounds beneficial for the human diet. Starting from the evidence that the
Stephanie P DiTroia et al.
Nature, 573(7773), 271-275 (2019-09-06)
Development is often assumed to be hardwired in the genome, but several lines of evidence indicate that it is susceptible to environmental modulation with potential long-term consequences, including in mammals1,2. The embryonic germline is of particular interest because of the
Manas R Gartia et al.
Scientific reports, 5, 16011-16011 (2015-11-04)
Limited training, high cost, and low equipment mobility leads to inaccuracies in decision making and is concerning with serious ocular injuries such as suspected ruptured globe or post-operative infections. Here, we present a novel point-of-service (POS) quantitative ascorbic acid (AA)
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Questions

1–2 of 2 Questions  

  1. What is the limit of detection for this assay?

    1 answer

    1. This assay is designed for the detection of ascorbic acid and is capable of detecting 0.01-10 nmol of ascorbic acid per assay.

      Helpful?

  2. What is the minimum detectable limit for your Ascorbic acid color metric assay kit. What is the accuracy level +/- ppm.

    1 answer

    1. While the absolute minimal limit has not been determined, this kit is intended and the standard curve is set up to detect 0.01-10 nmol of ascorbic acid per assay.

      Please see the link below to the product datasheet for further information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/590/655/mak074bul.pdf

      Helpful?

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