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MAK064

Sigma-Aldrich

Kits para análisis de lactato

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

Kit de ensayo del ácido láctico

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

aplicaciones

cosmetics
food and beverages

método de detección

colorimetric
fluorometric

enfermedades relevantes

endocrinological disorders, diabetes; cancer

temp. de almacenamiento

−20°C

Descripción general

El L(+)-lactato es un compuesto metabólico formado en animales por la acción de la enzima lactato deshidrogenasa.. El lactato se produce en las células en proliferación y durante condiciones anaerobias, como durante el ejercicio. Concentraciones anormalmente elevadas de lactato se han relacionado con enfermedades como el cáncer, la diabetes y la acidosis por lactato. El L(+)-lactato es el principal estereoisómero del lactato formado en el metabolismo intermediario humano y está presente en la sangre a concentraciones de alrededor de 1-2 mmol/l. Este kit proporciona un medio cómodo para detectar el L(+)-lactato en muestras biológicas. No es necesario el pretratamiento ni la purificación de las muestras

Aplicación

El kit del ensayo de lactato se ha utilizado para determinar las concentraciones de lactato en las muestras.[1][2]

Idoneidad

Adecuado para la detección de L(+)-lactato en medio de cultivo, medio de fermentación, sangre y células.

Principio

En este análisis, se determina la concentración de lactato mediante un ensayo enzimático, que da lugar a un producto colorimétrico (570 nm)/fluorométrico (λex = 535 nm/λem = 587 nm), proporcional al lactato presente. Los niveles típicos de sensibilidad para este kit oscilan entre 0,001 y 10 mM.

sustituido por

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H317,H334

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
8J15K06070
8A17K06070
8B23K06070
8L03K06070
8F30K06070

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Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
Prabha Shrestha et al.
PLoS pathogens, 13(9), e1006628-e1006628 (2017-09-19)
Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi's sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle
Bibi Uhre Nielsen et al.
PloS one, 15(9), e0238524-e0238524 (2020-09-12)
Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs)
Annasofia Anemone et al.
International journal of oncology, 51(2), 498-506 (2017-07-18)
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo
Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension.
Altara R, et al.
Frontiers in Pharmacology, 7, 436-436 (2016)
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Questions

1–9 of 9 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the formula for the MAK064D Lactate standard?

    1 answer

    1. The standard provided in the MAK064-1KT Lactate Assay Kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. It contains no other components or cofactors.

      Helpful?

  4. Since the L-(+)-Lactate standard is not a pure solution of sodium L-(+)-Lactate in water or buffer, which are the cofactors present in the standard?

    1 answer

    1. The standard provided in this kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. There are no other components or cofactors present in the standard.

      Helpful?

  5. What is the concentration range of lactate for this? The description says typical sensitivity is 0.001 - 10 mM, but in the product info the standard curve for colorimetric detection appears to go to a max. concentration of 0.0002µM (10nmol in 50µL sample)

    1 answer

    1. The lower limit of detection is calculated by dividing the lowest concentration standard by the maximum sample volume. The higher limit of detection is determined by dividing the highest concentration standard by the minimum sample volume.

      For the higher limit of detection, take the 10 nmol standard and divide by 1 uL to get 10 mM. For the lower limit of detection, take 0.02 nmol and divide by 50 uL to get 0.0004 mM. We have reached out to our vendor to discuss the range calculation further, as it seems there is some conflicting information.

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  6. Can this kit be used on conditioned media containing phenol red?

    1 answer

    1. If the sample being measured is the cell culture supernatant, the required volume is 20 uL. This is diluted with a total volume of 100 uL of the various kit reagents. The presence of phenol red may impact the results. It would be up to the end user to determine suitability.

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  7. On which enzymatic reaction is this kit based? Does pyruvate in the sample interfere with the assay?

    1 answer

    1. This kit utilizes lactate oxidase to measure lactate. In sufficient amounts pyrivate may impact the results. This can be corrected by subtracting any background from the blank value. See page 3 in the kit protocol provided in the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/411/763/mak064bul.pdf

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Can I make up my own Lactate standard to use with Product MAK064, Lactate Assay kit?

    1 answer

    1. Unfortunately, no. If you run the 100 mM L-(+)-Lactate standard (MAK064D) that comes with the kit, you should get good results. However, if you just use, for example, a solution of sodium L-(+)-lactate dissolved in water or buffer, you probably won't get good results. There are cofactors present in biological samples and in the L-(+)-Lactate standard that comes with the kit (which is not a pure solution of sodium L-(+)-Lactate in water or buffer). If such a "homemade" standard solution were to be spiked with a biological sample or the kit standard, the calculations would be more complicated, but the assay should work.

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