L8777 is recommended for cell culture because it is a sterile product.
L9379
Lectin from Phytolacca americana (pokeweed)
lyophilized powder
Sinónimos:
PWM, Pokeweed mitogen
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5 MG
US$ 236,00
10 MG
US$ 428,00
40 MG
US$ 1.300,00
US$ 236,00
PRECIOS SIN IMPUESTOS NACIONALES
Disponible para envío el11 de abril de 2025Detalles
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5 MG
US$ 236,00
10 MG
US$ 428,00
40 MG
US$ 1.300,00
About This Item
US$ 236,00
PRECIOS SIN IMPUESTOS NACIONALES
Disponible para envío el11 de abril de 2025Detalles
Productos recomendados
esterilidad
aseptically filled
Nivel de calidad
Formulario
lyophilized powder
potencia
≤0.3 μg/mL mitogenic activity
temp. de almacenamiento
2-8°C
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Categorías relacionadas
Descripción general
Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.[1]
Aplicación
Lectin from Phytolacca americana (pokeweed) has been used:
- as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation[2][3]
- as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes[4]
- as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures[5]
Acciones bioquímicas o fisiológicas
Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.[1]
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Forma física
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Nota de análisis
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Artículos
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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Is the product Pokeweed L9379 suitable for cell cultures from B lymphocytes? Or is better to use the product L8777? We culture bone marrow samples from B-cell dyscrasias for cytogenetic studies.
1 answer-
Helpful?
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how to prepare Pokeweed to be used as mitogen for B-ALL cytogenetics blood culture
1 answer-
This product can be dissolved in PBS and used at a concentration of up to 3 µg/mL for a mitogenic effect on human peripheral blood mononuclear cells. Concentrations higher than 3 µg/mL may be toxic to these cells.
Helpful?
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