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KEM0033

Sigma-Aldrich

T4 DNA Polymerase

Ultra-pure enzyme for nucleic acid modifications

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About This Item

grade

for molecular biology

assay

>99% (SDS-PAGE)

form

buffered aqueous solution

specific activity

5,555 U/mg

concentration

3,000 U/mL

shipped in

dry ice

storage temp.

−20°C

General description

T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′ → 3′ direction. This enzyme exhibits a powerful 3′ → 5′ exonuclease activity, while lacking any inherent 5′ → 3′ exonuclease or strand displacement functions.

Application

Suitable for DNA end-repair by fill-in of 5′ overhang and removal of 3′ overhang.

Features and Benefits

  • Ultra-purification process for ultimate enzyme performance
  • Highest quality specifications for ultimate product consistency
  • Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0042B (10X Blue Buffer)

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37° C.

Physical form

Supplied in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 6.5 @ 25° C.

Other Notes

Source of protein: Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.
Unit size: 2,000 U

Storage Class

10 - Combustible liquids

Certificados de análisis (COA)

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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing

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