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Merck

KEM0032

Sigma-Aldrich

Terminal deoxynucleotidyl Transferase (TdT)

Ultra-pure enzyme for nucleic acid modifications

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About This Item

grade

for molecular biology

assay

>99% (SDS-PAGE)

form

buffered aqueous solution

specific activity

27,400 U/mg

concentration

20,000 U/mL

shipped in

dry ice

storage temp.

−20°C

General description

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxyl terminus of single or double stranded DNA molecules. The presence of 1 mM Co2+ stimulates the tailing of the 3′-ends of DNA fragments. This construct is sold as an N-terminal truncation of the terminal transferase gene attached to an N-terminal fusion tag.

Application

Suitable for:
  • Homopolymeric tailing to the 3′ OH
  • TUNEL assay
  • 5′-RACE
  • Labeling of 3′ ends

Features and Benefits

  • Ultra-purification process for ultimate enzyme performance
  • Highest quality specifications for ultimate product consistency
  • Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0043B (10X Green Buffer)KEM0045B (2.5 mM CoCl2

Unit Definition

1 unit is defined as the amount of polymerase required to convert 1 nmol of dTTPs into acid insoluble material in 1 hour at 37° C.

Physical form

Supplied in 50 mM KPO4, 100 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% glycerol at pH 7.3 @ 25° C.

Other Notes

Source of protein: An E. coli strain that carries the cloned terminal transferase gene from calf thymus.
Unit size: 6,000 U

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing

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