G9637

Glycerol 3-phosphate Oxidase from Pediococcus sp.

lyophilized powder, 40-80 units/mg solid, pH 8.1

• Sinónimos: sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase
• Número de CAS: 9046-28-0
• Comisión internacional de enzimas: 1.1.3.21 (BRENDA, IUBMB)
• Número CE: 232-932-0
• Número MDL: MFCD00131209
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bacterial (Pediococcus spp.)
• Nivel de calidad: 200
• Formulario: lyophilized powder
• actividad específica: 40-80 units/mg solid, pH 8.1
• mol peso: ~76 kDa (gel filtration)
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate.[1] Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa.[2] It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.[3]

Aplicación

Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver[4] and in immobilization studies on pencil graphite (PG) electrode.[5]

This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.

Propiedades físicas

Isoelectric point : 4.1 -/+0.1
Michaelis constants : 3.2x10 ^-3M (L-α-Glycerophosphate), 6.8 x 10 ^-3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg⁺⁺, Ag⁺
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40o^C
pH Stability : pH 6.5 - 8.5 (25o^C, 20hr) Thermal stability : below 40oC (pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate

Definición de unidad

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H₂O₂ per min at 37°C, at the appropriate pH.

Forma física

Lyophilized powder containing stabilizers

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)