F3543

Fluorescent Brightener 28

used as a stain and brightening agent

• Sinónimos: Calcofluor White M2R, Tinopal UNPA-GX
• Fórmula empírica (notación de Hill): C₄₀H₄₄N₁₂O₁₀S₂
• Número de CAS: 4404-43-7
• Peso molecular: 916.98
• EC Number: 224-548-7
• MDL number: MFCD00308955
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894849
• NACRES: NA.47

Propiedades

• form: powder
• mp: 300 °C
• εmax: 40-60 at 238-242 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OCCN(CCO)c1nc(Nc2ccccc2)nc(Nc3ccc(\C=C\c4ccc(Nc5nc(Nc6ccccc6)nc(n5)N(CCO)CCO)cc4S(O)(=O)=O)c(c3)S(O)(=O)=O)n1
• InChI: 1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+
• InChI key: CNGYZEMWVAWWOB-VAWYXSNFSA-N

Descripción

General description

Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps. It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.

Fluorescent Brightener 28 has been used for the staining of fungal cell walls. It has also been used for the staining of Candida albicans biofilm.

Application

It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves