Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

E6779

Millipore

EZview™ Red Anti-HA Affinity Gel

Sinónimos:

Anti-HA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
12352203
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad

caducidad

1 yr at 2‑8 °C

clases químicas de analitos

proteins (hemagglutinin)

técnicas

affinity chromatography: suitable
immunoprecipitation (IP): suitable

Matriz

crosslinked agarose

capacidad

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

Especificidad

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Aplicación

EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis[1] and immunoprecipitation assay.[2]
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Características y beneficios

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Otras notas

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Forma física

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Información legal

EZview is a trademark of Sigma-Aldrich Co. LLC

Producto relacionado

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000373135
0000360953
0000353120
0000343116
0000307406

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Activation of c-Abl kinase potentiates the anti-myeloma drug lenalidomide by promoting DDA1 protein recruitment to the CRL4 ubiquitin ligase
Gao S, et al.
The Journal of biological chemistry, 292(9), 3683-3691 (2017)
Novel Mitochondrial Substrates of Omi Indicate a New Regulatory Role in Neurodegenerative Disorders
Johnson,F et al.
PLoS Biology, 4(9) (2009)
The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis.
Chen YL, Capeyrou R, Humbert O, et al.
Nucleic Acids Research, 42(11), 7330-7345 (2014)
The nonreceptor tyrosine kinase c-Abl phosphorylates Runx1 and regulates Runx1-mediated megakaryocyte maturation
Liu H, et al.
Biochimica et biophysica acta. General subjects, 1865(8), 1060-1072 (2018)
Yuanyuan Wang et al.
Nucleic acids research, 45(20), 11594-11606 (2017-10-17)
DNA N6-methyladenine (6mA) is newly rediscovered as a potential epigenetic mark across a more diverse range of eukaryotes than previously realized. As a unicellular model organism, Tetrahymena thermophila is among the first eukaryotes reported to contain 6mA modification. However, lack
Ver todos los artículos relacionados

Contenido relacionado

Expresión proteica

Tecnologías de expresión de proteínas para varios sistemas de expresión que propician la investigación, la terapéutica y la producción de vacunas.

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

Questions

Reviews

No rating value

Active Filters

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico