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D5921

Sigma-Aldrich

DMEM - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

DME, DMEM

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About This Item

MDL number:
MFCD00217342
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medio de Eagle modificado de Dulbecco, poca glucosa, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:
  • living colon cancer SW620 cells
  • human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
  • mammal and canine derived adipose-derived mesenchymal stem cells

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

also commonly purchased with this product

Referencia del producto
Descripción
Precios

supplement

Referencia del producto
Descripción
Precios

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Fabian E Ortega et al.
eLife, 8 (2019-02-06)
Listeria monocytogenes hijacks host actin to promote its intracellular motility and intercellular spread. While L. monocytogenes virulence hinges on cell-to-cell spread, little is known about the dynamics of bacterial spread in epithelia at a population level. Here, we use live
Christina Schmid et al.
Journal of pharmacological and toxicological methods, 106, 106915-106915 (2020-09-02)
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are being evaluated for their use in pharmacological and toxicological testing, particularly for electrophysiological side effects. However, little is known about the composition of the commercially available iCell cardiomyocyte (Fuijifilm Cellular Dynamics) cultures
Kenji Ohe et al.
The Journal of steroid biochemistry and molecular biology, 182, 21-26 (2018-04-22)
The high-mobility group A protein 1a (HMGA1a) protein is known as an oncogene whose expression level in cancer tissue correlates with the malignant potential, and known as a component of senescence-related structures connecting it to tumor suppressor networks in fibroblasts.
C Mignon et al.
Scientific reports, 7(1), 2797-2797 (2017-06-07)
Photobiomodulation-based (LLLT) therapies show tantalizing promise for treatment of skin diseases. Confidence in this approach is blighted however by lamentable inconsistency in published experimental designs, and so complicates interpretation. Here we interrogate the appropriateness of a range of previously-reported treatment
Giuseppe Antonacci et al.
Scientific reports, 6, 37217-37217 (2016-11-16)
Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and
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