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Merck

D4010

Sigma-Aldrich

2′-Deoxyguanosine 5′-triphosphate sodium salt hydrate

≥96% (HPLC)

Sinónimos:

2′-deoxy-, 5′-triphosphate sodium salt, Guanosine, dGTP sodium salt hydrate

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About This Item

Fórmula empírica (notación de Hill):
C10H16N5O13P3 · xNa+ · yH2O
Número de CAS:
Peso molecular:
507.18 (anhydrous free acid basis)
UNSPSC Code:
41106305
PubChem Substance ID:
NACRES:
NA.51

biological source

synthetic (organic)

assay

≥96% (HPLC)

form

powder

shipped in

dry ice

storage temp.

−20°C

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Categorías relacionadas

Application

2′-Deoxyguanosine 5′-triphosphate sodium salt hydrate has been used:
  • as a component in a polymerase chain reaction (PCR) mixture for PCR product formation using AM-toxin primers
  • as an internal standard to improve the analytical performance of capillary electrophoresis (CE) analysis for the evaluation of the cleanup process
  • as a putative substrate in in vitro enzyme activity assay to incubate with nudix hydrolase 15 (NUDT15) protein for the evaluation of putative NUDT15 inhibitors

Biochem/physiol Actions

Deoxyguanosine 5′-triphosphate (dGTP), a purine deoxynucleotide, is a substrate of DNA polymerase(s) and reverse transcriptases. dGTP is useful to study the distribution, specificity, and kinetics of various nucleoside-triphosphatases (NTPase) and ribonucleotide reductases.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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During DNA synthesis, DNA polymerases must select against ribonucleotides, present at much higher levels compared with deoxyribonucleotides. Most DNA polymerases are equipped to exclude ribonucleotides from their active site through a bulky side chain residue that can sterically block the
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Analytical biochemistry, 335(1), 150-161 (2004-11-03)
A novel approach for quantitation of DNA (oligonucleotides) with an unprecedented accuracy of approximately 1% is reported. Quantitation of DNA is commonly performed by measuring UV absorption or fluorescence from dyes intercalated into DNA. Both methods need accurate quantitation standards
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Proceedings of the National Academy of Sciences of the United States of America, 108(24), 9815-9820 (2011-06-02)
Human ribonucleotide reductases (hRNRs) catalyze the conversion of nucleotides to deoxynucleotides and are composed of α- and β-subunits that form active α(n)β(m) (n, m = 2 or 6) complexes. α binds NDP substrates (CDP, UDP, ADP, and GDP, C site)
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Ganciclovir (GCV) is the first-line therapy against human cytomegalovirus (HCMV), a widespread infection that is particularly dangerous for immunodeficient individuals. Closely resembling deoxyguanosine triphosphate, the tri-phosphorylated metabolite of GCV (GCV-TP) is preferentially incorporated by the viral DNA polymerase, thereby terminating

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