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D2532

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Sinónimos:

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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Talla/SKUDisponibilidadPrecio
5 mL
Comprobar disponibilidad del carrito
US$ 213,00
5 x 5 mL
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US$ 724,00

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NACRES:
NA.25
UNSPSC Code:
12161700

US$ 213,00

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Quality Segment

form

solution

technique(s)

Southern blotting: suitable, nucleic acid detection: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

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Este artículo
D9905P1415H7140
description

for Northern and Southern blotting, solution

description

for Northern and Southern blotting, lyophilized powder

description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, liquid (2x concentrate)

technique(s)

Southern blotting: suitable

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

-

Quality Level

300

Quality Level

100

Quality Level

100, 200

Quality Level

100

form

solution

form

lyophilized powder

form

liquid (2x concentrate)

form

liquid (2x concentrate)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-10 to -25°C

storage temp.

−20°C

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected


Clase de almacenamiento

12 - Non Combustible Liquids

¿Qué está pasando?

WGK 3

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable



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Questions

  1. Can you share the CAS # for the components (Ficoll and PVP) that are used to make the 50X Denhart's Solution?

    1 answer
    1. The CAS number for the Ficoll ingredient is 26873-85-8. The CAS number for the PVP ingredient is 9003-39-8 2.

      Below is the link to the Product Data Sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/172/670/d2532dat.pdf

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